Fig 1: Complement-cascade proteins are co-expressed with endothelial cell markers in ONX-0914-treated mice.Proteomic analysis of C3aR1 in psoas of 6 m BlAJ, 12 m BlAJ and BlAJ+ONX, 12 m C57Bl mice (A). Evaluation of C3 and C5 concentration in the serum of 6 m BlAJ, 12 m BlAJ and BlAJ+ONX, 12 m C57Bl mice (B). Representative images and quantification of psoas muscles from 6 m BlAJ, 12 m BlAJ and BlAJ+ONX, 12 m C57Bl with big vessels co-stained with αSMA (in green) and C3aR1/CD88/CD93 (in red). Nuclei were stained in DAPI (blue) (scale bar: 100 μm) (C). Representative images and quantification of psoas muscles from 6 m BlAJ, 12 m BlAJ and BlAJ+ONX, 12 m C57Bl with small vessels co-stained with isolectin (in green) and C3aR1/CD88/CD93 (in red). Nuclei were stained in DAPI (blue) (scale bar: 50 μm) (D). Data are presented as mean ± SD of n = 3 independent experiments with n = 3–6 animals/group. One-way ANOVA, Tukey multiple comparison test for WB and non-parametric test followed by Kruskal–Wallis test for vessels’ count: *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.